bio_ens_hit.pm

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=head1 LICENSE
 
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you may not use this file except in compliance with the License.
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     http://www.apache.org/licenses/LICENSE-2.0
 
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=cut
 
 
=head1 CONTACT
 
  Please email comments or questions to the public Ensembl
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=cut
 
=head1 DESCRIPTION
 
Sequence alignment hits were previously stored within the core database
as ungapped alignments. This imposed 2 major constraints on alignments:
 
  a) alignments for a single hit record would require multiple rows in
     the database, and
  b) it was not possible to accurately retrieve the exact original alignment.
 
Therefore, in the new branch sequence alignments are now stored as
ungapped alignments in the cigar line format (where CIGAR stands for
Concise Idiosyncratic Gapped Alignment Report).
 
In the cigar line format alignments are sotred as follows:
 
  M: Match
  D: Deletino
  I: Insertion
 
An example of an alignment for a hypthetical protein match is shown
below:
 
 
  Query:   42 PGPAGLP----GSVGLQGPRGLRGPLP-GPLGPPL...
              PG    P    G     GP   R      PLGP
  Sbjct: 1672 PGTP*TPLVPLGPWVPLGPSSPR--LPSGPLGPTD...
 
protein_align_feature table as the following cigar line:
 
  7M4D12M2I2MD7M
 
=cut
 
package Bio::EnsEMBL::Utils::Converter::bio_ens_hit;
 
use strict;
use vars qw(@ISA);
use Bio::EnsEMBL::Utils::Converter::bio_ens;
use Bio::EnsEMBL::DnaDnaAlignFeature;
use Bio::EnsEMBL::DnaPepAlignFeature;
use Bio::EnsEMBL::PepDnaAlignFeature;
use Bio::EnsEMBL::ProteinFeature;
 
@ISA = qw(Bio::EnsEMBL::Utils::Converter::bio_ens);
 
sub _initialize {
    my ($self, @args) = @_;
    $self->SUPER::_initialize(@args);
 
    # After super initialized, analysis and contig are ready.
    my $bio_ens_seqFeature_converter = new Bio::EnsEMBL::Utils::Converter(
        -in => 'Bio::SeqFeature::Generic',
        -out => 'Bio::EnsEMBL::SeqFeature',
        -analysis => $self->analysis,
        -contig => $self->contig
    );
    $self->_bio_ens_seqFeature_converter($bio_ens_seqFeature_converter);
 
}
 
sub _convert_single {
    my ($self, $input) = @_;
    
    my $in = $self->in;
    my $out = $self->out;
    
    if($in =~ /Bio::Search::Hit::GenericHit/){
        return $self->_convert_single_hit($input);
    }elsif($in =~ /Bio::Search::HSP::GenericHSP/){
        return $self->_convert_single_hsp($input);
    }else{
        $self->throw("[$in]->[$out], not implemented");
    }
}
 
sub _convert_single_hit {
 
 
}
 
sub _convert_single_hsp {
    my ($self, $hsp) = @_;
 
    unless(ref($hsp) && $hsp->isa('Bio::Search::HSP::GenericHSP')){
        $self->throw("a GenericHSP object needed");
    }
 
    my $bio_ens_seqFeature_converter = $self->_bio_ens_seqFeature_converter;
    my $ens_feature1 = $bio_ens_seqFeature_converter->_convert_single(
        $hsp->feature1);
    my $ens_feature2 = $bio_ens_seqFeature_converter->_convert_single(
        $hsp->feature2);
 
    $ens_feature1->p_value($hsp->evalue);
    $ens_feature1->score($hsp->score);
    $ens_feature1->percent_id($hsp->percent_identity);
    $ens_feature2->p_value($hsp->evalue);
    $ens_feature2->score($hsp->score);
    $ens_feature2->percent_id($hsp->percent_identity);
    
    my $cigar_string = $hsp->cigar_string;
    my @args = (
        -feature1 => $ens_feature1,
        -feature2 => $ens_feature2,
        -cigar_string => $cigar_string
    );
 
    my $contig = $self->contig;
    # choose the AlignFeature based on the blast program
    my $program = $hsp->algorithm;
 
    $self->throw("HSP does not have algorithm value") unless(defined($program));
    my $align_feature;
    
    if($program =~ /blastn/i){
        $align_feature = new Bio::EnsEMBL::DnaDnaAlignFeature(@args);
        $align_feature->attach_seq($contig);
    }elsif($program =~ /blastx/i){
        $align_feature = new Bio::EnsEMBL::DnaPepAlignFeature(@args);
        $align_feature->attach_seq($contig);
    }else{
        $self->throw("$program is not supported yet");
    }
    
    return $align_feature;
}
 
# an internal getter/setter for a converter used for seq feature conversion.
 
sub _bio_ens_seqFeature_converter {
    my ($self, $arg) = @_;
    if(defined $arg){
        $self->{_bio_ens_seqFeature_converter} = $arg;
    }
    return $self->{_bio_ens_seqFeature_converter};
}
 
1;