TranscriptFactory.pm

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=head1 LICENSE
 
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Licensed under the Apache License, Version 2.0 (the "License");
you may not use this file except in compliance with the License.
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     http://www.apache.org/licenses/LICENSE-2.0
 
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=cut
 
 
=head1 CONTACT
 
  Please email comments or questions to the public Ensembl
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=cut
 
=head1 NAME
 
Bio::EnsEMBL::TranscriptFactory - Module having the fset2transcript*
subroutines
 
=head1 SYNOPSIS
 
  use Bio::EnsEMBL::TranscriptFactory;
 
  &Bio::EnsEMBL::TranscriptFactory::fset2transcript($fset_id);
 
=head1 DESCRIPTION
 
Module containing the subroutines fset2transcript*, 
which create transcripts from features (formally housed in
Bio::EnsEMBL::DBSQL::Utils).
 
=head1 METHODS
 
=cut
 
package Bio::EnsEMBL::TranscriptFactory;
 
use strict;
 
use Bio::EnsEMBL::Exon;
use Bio::EnsEMBL::Translation;
use Bio::EnsEMBL::Transcript;
 
sub fset2transcript {
    my ($genscan,$contig)=@_;
 
  
    unless ($genscan->isa ("Bio::EnsEMBL::SeqFeatureI"))
    {print "$genscan must be Bio::EnsEMBL::SeqFeatureI\n";}
     
    my $transcript = new Bio::EnsEMBL::Transcript;
    $transcript->temporary_id($contig->id . "." . $genscan->seqname);
        
    my @exons;
    my $count= 1;
    
    foreach my $f ($genscan->sub_SeqFeature) {
  
    my $exon  = new Bio::EnsEMBL::Exon;
    $transcript->add_Exon($exon);
        $exon->contig   ($contig);
    $exon->start    ($f->start);
    $exon->end      ($f->end  );
    $exon->strand   ($f->strand);
    $exon->phase    ($f->phase);
    $exon->end_phase( ($exon->phase + $exon->length)%3 );
    #$exon->score($f->score);
#   $exon->p_value($f->p_value);
    $exon->slice($contig->primary_seq);
    
    push(@exons,$exon);
    $count++;
    
    }
    
    if( $count == 1 ) {
    $genscan->throw("Got a 0 exon genscan");
    }
 
    my $translation = new Bio::EnsEMBL::Translation;
    #
    # This code got changed due to Translation convention changing. Should work...
    #
    
    if ($exons[0]->strand == 1) {
    @exons = sort {$a->start <=> $b->start} @exons;
    } else {
    @exons = sort {$b->start <=> $a->start} @exons;
    }
    
    $translation->start(1);
    $translation->end($exons[scalar(@exons)-1]->length);
    
    $translation->start_Exon($exons[0]);
    $translation->end_Exon($exons[$#exons]);
 
    my $endphase = $exons[0]->end_phase;
    
    foreach my $exon (@exons) {
    
      if ( $exon == $exons[0] ){
    next;
      }
      $exon->phase         ($endphase);
      $endphase = $exon->end_phase;
    }
    
    $transcript->translation($translation);
    
    return $transcript;
}
 
sub fset2transcript_guess_phases {
    my ($fset,$contig) = @_;
 
    my $transcript = new Bio::EnsEMBL::Transcript;
 
    $transcript->temporary_id($contig->id . "." . $fset->id);
 
 
    my @exons;
    my $count    = 1;
 
    foreach my $f ($fset->sub_SeqFeature) {
 
    my $exon  = new Bio::EnsEMBL::Exon;
        $exon->contig   ($contig);
    $exon->start    ($f->start);
    $exon->end      ($f->end  );
    $exon->strand   ($f->strand);
    #$exon->score($f->score);
#   $exon->p_value($f->p_value);
    $exon->slice($contig);
    $exon->phase($f->phase); 
    push(@exons,$exon);
    $count++;
    
    }
    
    my $translation = new Bio::EnsEMBL::Translation;
    
    if ($exons[0]->strand == 1) {
    @exons = sort {$a->start <=> $b->start} @exons;
    } else {
    @exons = sort {$b->start <=> $a->start} @exons;
    }
    
    $translation->start        (1);
    $translation->end          ($exons[$#exons]->end - $exons[$#exons]->start + 1);
    $translation->start_Exon($exons[0]);
    $translation->end_Exon($exons[$#exons]);
    $transcript->translation($translation);
    
    my $endphase = 0;
    
    foreach my $exon (@exons) {
    
    $exon      ->phase   ($endphase);
    $transcript->add_Exon($exon);
 
    $endphase = $exon->end_phase(($exon->phase + $exon->length)%3);
    
    }
 
 
    if ($transcript->translate->seq !~ /\*/) {
    return $transcript;
    }   
 
    $endphase = 1;
    
    foreach my $exon (@exons) {
    $exon->phase($endphase);
    $endphase = $exon->end_phase(($exon->phase + $exon->length)%3);
    }
 
    if ($transcript->translate->seq !~ /\*/) {
    return $transcript;
    }   
 
    $endphase = 2;
    
    foreach my $exon (@exons) {
    $exon->phase($endphase);
    $endphase = $exon->end_phase(($exon->phase + $exon->length)%3);
    }
    
    if ($transcript->translate->seq !~ /\*/) {
    return $transcript;
    }   
}
 
sub fset2transcript_3frame {
  my ($fset,$contig) = @_;
 
  my @f = $fset->sub_SeqFeature;
  
  if ($f[0]->strand == 1) {
    @f = sort {$a->start <=> $b->start} @f;
  } else {
    @f = sort {$b->start <=> $a->start} @f;
  }
 
  my @transcripts;
 
  my $startphase = 0;
 
  while ($startphase < 3) {
    my $endphase = $startphase;
 
    my $transcript = new Bio::EnsEMBL::Transcript;
 
    push(@transcripts,$transcript);
 
    $transcript->temporary_id($contig->id . "." . $endphase);
 
    my $count    = 1;
    my @exons;
 
   
    foreach my $f (@f) {
      #print "exon seqname = ".$f->seqname."\n";
      my $exon  = new Bio::EnsEMBL::Exon;
      #print STDERR "exon ".$f->gffstring."\n";
      push(@exons,$exon);
      $exon->seqname($f->seqname);
      $exon->temporary_id ($contig->id . ".$count");
      $exon->contig   ($contig);
      $exon->start    ($f->start);
      $exon->end      ($f->end  );
      $exon->strand   ($f->strand);
      $exon->slice($contig);
      $exon->phase    ($endphase);
      $exon->end_phase( ($exon->phase + $exon->length)%3 );
      #$exon->score    ($f->score);
#      $exon->p_value  ($f->p_value);
      $endphase = $exon->end_phase;
 
      $transcript->add_Exon($exon);
      $count++;
 
      #print STDERR "Added exon start " . $exon->start . " end " . $exon->end . " strand " . $exon->strand . " score " . $exon->score . " pvalue " . $exon->p_value . "\n";
    }
       
    my $translation = new Bio::EnsEMBL::Translation;
 
    my $contig_id = "";
    my $fset_id   = "";
 
    if (defined($contig->id)) {
       $contig_id = $contig->id;
    }
    if (defined($fset->id)) {
       $fset_id = $fset->id;
    }
 
    $translation->temporary_id($contig_id . "." . $fset_id);
    $translation->start        (1);
    $translation->end          ($exons[$#exons]->end - $exons[$#exons]->start + 1);
    $translation->start_Exon($exons[0]);
    $translation->end_Exon  ($exons[$#exons]);
    $transcript->translation($translation);
 
 #  print STDERR "Phase $startphase " . $transcript->translate->seq . "\n";
 
    $startphase++;
  }
  #print "finshed  fset2transcript_3frame\n";
  return @transcripts;
}
 
 
sub fset2transcript_with_seq {
    my ($genscan,$seq)=@_;
 
  
    unless ($genscan->isa ("Bio::EnsEMBL::SeqFeatureI"))
    {print "$genscan must be Bio::EnsEMBL::SeqFeatureI\n";}
    unless ($seq->isa ("Bio::PrimarySeqI") || $seq->isa ("Bio::SeqI"))
    {print "$seq must be Bio::SeqI or a Bio::PrimarySeqI\n";}
 
    #print STDERR "running fset2transcript\n";
    my $transcript = new Bio::EnsEMBL::Transcript;
    $transcript->temporary_id($seq->id . "." . $genscan->seqname);
        
    my @exons;
    my $count= 1;
    
    foreach my $f ($genscan->sub_SeqFeature) {
  
    my $exon  = new Bio::EnsEMBL::Exon;
        $exon->contig   ($seq);
    $exon->start    ($f->start);
    $exon->end      ($f->end  );
    $exon->strand   ($f->strand);
    $exon->phase    ($f->phase);
    $exon->end_phase( ($exon->phase + $exon->length)%3 );
    #$exon->score ($f->score);
    #print STDERR "contig is a = ".$seq."\n";
    $exon->slice($seq);
    
    push(@exons,$exon);
    $count++;
    
    }
 
    foreach my $exon (@exons) {
        
      $transcript->add_Exon($exon);
    
    
    }
    return $transcript;
   
}
 
 
 
1;