CodelinkParser.pm

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=head1 LICENSE
 
See the NOTICE file distributed with this work for additional information
regarding copyright ownership.
 
Licensed under the Apache License, Version 2.0 (the "License");
you may not use this file except in compliance with the License.
You may obtain a copy of the License at
 
     http://www.apache.org/licenses/LICENSE-2.0
 
Unless required by applicable law or agreed to in writing, software
distributed under the License is distributed on an "AS IS" BASIS,
WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
See the License for the specific language governing permissions and
limitations under the License.
 
=cut
 
package XrefParser::CodelinkParser;
 
use strict;
use File::Basename;
use Carp;
use base qw( XrefParser::BaseParser );
 
# Parser for Codelink probes
 
#>GE469530
#TTGTTTTCAGCTTGCTTCTGTCATTCTTCC
#>GE469548
#CACAGTTGGGTGAAGCTGGTGATGAAGGTA
 
sub run {
 
  my ($self, $ref_arg) = @_;
  my $source_id    = $ref_arg->{source_id};
  my $species_id   = $ref_arg->{species_id};
  my $files        = $ref_arg->{files};
  my $release_file = $ref_arg->{rel_file};
  my $verbose      = $ref_arg->{verbose};
 
  if((!defined $source_id) or (!defined $species_id) or (!defined $files) or (!defined $release_file)){
    croak "Need to pass source_id, species_id, files and rel_file as pairs";
  }
  $verbose |=0;
 
  my $file = @{$files}[0];
 
  my @xrefs;
 
  local $/ = "\n>";
 
  my $codelink_io = $self->get_filehandle($file);
  if ( !defined $codelink_io ) {
    print STDERR "ERROR: Could not open $file\n";
    return 1;    # 1 = error
  }
 
  while ( $_ = $codelink_io->getline() ) {
    my $xref;
 
    my ($header, $sequence) = $_ =~ /^>?(.+?)\n([^>]*)/s or warn("Can't parse FASTA entry: $_\n");
 
    # deconstruct header - only accession for now
    my $accession = $header;
 
    # make sequence into one long string - probably not necessary for short probes
    $sequence =~ s/\n//g;
 
    # build the xref object and store it
    $xref->{ACCESSION}     = $accession;
    $xref->{LABEL}         = $accession;
    $xref->{SEQUENCE}      = $sequence;
    $xref->{SOURCE_ID}     = $source_id;
    $xref->{SPECIES_ID}    = $species_id;
    $xref->{SEQUENCE_TYPE} = 'dna';
    $xref->{STATUS}        = 'experimental';
 
    push @xrefs, $xref;
 
  }
 
  $codelink_io->close();
 
 
  $self->upload_xref_object_graphs(\@xrefs);
 
  print scalar(@xrefs) . " Codelink xrefs succesfully parsed\n" if($verbose);
 
  return 0; #successful
}
 
1;